Hybrid corn variety X21325

ABSTRACT

The invention provides seed and plants of the hybrid corn variety designated X21325. The invention thus relates to the plants, seeds and tissue cultures of the variety X21325, and to methods for producing a corn plant produced by crossing a corn plant of variety X21325 with itself or with another corn plant, such as a plant of another variety. The invention further relates to genetic complements of plants of variety X21325.

This application claims a priority based on provisional application62/185,002 which was filed in the U.S. Patent and Trademark Office onJun. 26, 2015, the entire disclosure of which is hereby incorporated byreference.

FIELD OF THE INVENTION

This invention is in the field of corn breeding. In particular, theinvention relates to a hybrid corn variety designated X21325 thatincludes plants and seeds of hybrid corn variety.

BACKGROUND OF THE INVENTION

Corn (Zea mays L.) is the most important and abundant crop produced inthe United States. Corn is used as human food, livestock feed, and as anindustrial raw material. The food uses of corn include kernels for humanconsumption, dry milling products such as grits, meal and flour, and wetmilling products such as corn starch, corn syrups, and dextrose. Cornoil recovered from corn germ is a by-product of both dry and wet millingindustries. Both grain and non-grain portions of corn plants are usedextensively as livestock feed, primarily for beef cattle, dairy cattle,hogs, and poultry.

Corn is used to produce ethanol while corn starch and flour are used inthe paper and textile industries. Corn is also used in adhesives,building materials, foundry binders, laundry starches, explosives,oil-well muds, and other mining applications. Plant parts other than thegrain of corn are also used in industry; for example, stalks and husksare made into paper and wallboard and cobs are used for fuel and to makecharcoal.

The goal of a corn breeder is to improve a corn plant's performance andtherefore, its economic value by combining various desirable traits intoa single plant. Improved performance is manifested in many ways. Higheryields of corn plants contribute to a more abundant food supply, a moreprofitable agriculture and a lower cost of food products for theconsumer. Improved quality makes corn kernels more nutritious. Improvedplant health increases the yield and quality of the plant and reducesthe need for application of protective chemicals. Adapting corn plantsto a wider range of production areas achieves improved yield andvegetative growth. Improved plant uniformity enhances the farmer'sability to mechanically harvest corn.

Natural, or open pollination, occurs in corn when wind blows pollen fromthe tassels to the silks that protrude from the tops of the ear shootand may include both self- and cross-pollination. Vigor is restored whentwo different inbred lines are cross-pollinated to produce the firstgeneration (F₁) progeny. A cross between two defined homozygous inbredcorn plants produce a uniform population of heterozygous hybrid cornplants and such hybrid corn plants are capable of being generatedindefinitely from the corresponding inbred seed supply.

When two different, unrelated inbred corn parent plants are crossed toproduce an F₁ hybrid, one inbred parent is designated as the male, orpollen parent, and the other inbred parent is designated as the female,or seed parent. Because corn plants are monoecious, hybrid seedproduction requires elimination or inactivation of pollen produced bythe female parent to render the female parent plant male sterile. Thisserves to prevent the inbred corn plant designated as the female fromself-pollinating.

The development of hybrid corn plants is a slow, costly process thatrequires the expertise of breeders and many other specialists. Thedevelopment of new hybrid corn varieties in a corn plant breedingprogram involves numerous steps, including: (1) selection of parent cornplants (germplasm) for initial breeding crosses; (2) inbreeding of theselected plants from the breeding crosses for several generations toproduce a series of inbred lines, which individually breed true and arehighly uniform; and, (3) crossing a selected inbred line with anunrelated line to produce the F₁ hybrid progeny having restored vigor.

Because hybrid corn varieties lose their commercial competitiveness overtime, a continuing need exists for novel hybrid corn varieties withimproved characteristics. To protect and to enhance yield production,trait technologies and seed treatment options provide additional cropplan flexibility and cost effective control against insects, weeds anddiseases, thereby further enhancing the potential of hybrid corn varietyX21325.

DETAILED DESCRIPTION OF THE INVENTION I. Definitions of PlantCharacteristics

In the description and examples that follow, a number of terms are used.To provide a clear and consistent understanding of the specification andclaims, including the scope to be given such terms, the followingdefinitions are provided.

Anther Color: Recorded at the time of pollen shed when anthers areactively dehiscing pollen as a standard color name [Light Green (1),Green-Yellow (5), Pale Yellow (6), Yellow (7), Salmon (9), Pink (11),Cherry Red (13), Purple (17), Tan (22)] and Munsell color code.

Anthocyanin in Brace Roots: Relative rating of the expression ofanthocyanin in the brace roots (none, faint, 50% purpling/moderate, anddark) recorded two weeks after flowering.

Biomass: Total amount of above-ground plant material harvested, measuredin tons per acre adjusted for 65% moisture.

Cob Color: Recorded as a standard color name [Pink-Orange (10), Pink(11), Light Red (12), Cherry Red (13), Red (14), White (19)] and Munsellcolor code.

Crude protein: Available and unavailable protein; measured as totalnitrogen multiplied by 6.25 and as a percentage of total dry matter.

Ears Per Stalk: Number of ears with seed set on each plant.

Ear Height: Distance measured in centimeters from the ground to thehighest placed ear node (attachment point of the ear shank) of theuppermost developed ear on the stalk.

Ear Leaves: One or more distinct ear leaves on ear husks at floweringrepresented as present or absent. This may be environmentallyinfluenced, and is recorded as present when ear leaves are present insufficient size (>0.25 inches) and on several plants in the middle ofthe row.

Ear Length: Length of an unhusked ear from the butt to the tip; measuredin centimeters.

Ear-Node Leaf Length: Length of the ear-node leaf; measured incentimeters.

Ear-Node Leaf Width: Width of the ear-node leaf at its widest point;measured in centimeters.

Ear Taper: Relative taper of the unshelled ear; rated as Slight ornearly straight, Average, or Extreme or conical.

Endosperm Type: Region of the kernel between the germ and the seed coat;rated as sweet, extra sweet (sh2), normal starch, high amylase starch,waxy, high protein, high lysine, supersweet (se), high oil andother-specify.

Environments: Number of different geographic locations where two hybridsare grown together and in the same experiment.

GDU (Growing degree units): Number of heat units accumulated over timecalculated using the Barger Method, wherein the heat units for each24-hour period are: [(Max. temp+Min. temp)/2]−50, with 86° F. thehighest maximum temperature used and 50° F. the lowest minimumtemperature used.

Genotype: Genetic constitution of a plant, plant part, or cell.

Glume Color: Color of the glume after exposure to sunlight and justbefore extruding anthers; recorded as a standard color name [Light Green(1), Medium Green (2), Dark Green (3), Very Dark Green (4), Green-Yellow(5), Salmon (9), Pink (11), Cherry Red (13), Red (14), Pale Purple (16)]and Munsell color code.

Hard Endosperm Color: Color of the region of the endosperm between thefloury endosperm and the aleurone layer in yellow dent corn; recorded asa standard color name [Pale Yellow (6), Yellow (7), Yellow-Orange (8),Orange (9), Purple (17), White (19), Tan (22)] and a Munsell color code.

Kernel Crown Color: Color of the portion of the kernel distal to the tipcap; recorded as a standard color name [Pale Yellow (6), Yellow (7),Yellow-Orange (8), Orange (9), White (19)] and Munsell color code.

Kernel Row Alignment: Alignment of kernels scored as straight, slightlycurved, or spiral as determined by standing the unshelled ear on itsbase and looking down at the tip.

Kernel Row Type: Presence (Distinct) or absence (Indistinct) of definedkernel rows.

Kernel Type: Two of the common types for field corn are Flint and Dent;where Flint has mostly hard, glassy endosperm with smooth, hard seedcoats, and Dent has soft cores of starch that cause the end of thekernels to collapse or dent during drying.

Lateral Tassel Branches: Number of primary lateral tassel branches thatoriginate from the central spike.

Leaf Angle: Horizontal (less than 30 degrees), Semi-Erect (30 to 60degrees), Erect (greater than 60 degrees); measured on the upper half ofthe plant as the angle between a line perpendicular to the stalk and theunderside of a leaf at its base.

Leaf Color: Color of the leaf; recorded as standard color name [LightGreen (1), Medium Green (2), Dark Green (3), Very Dark Green (4)] andMunsell color code.

Leaves Above Ear: Number of leaves above the ear leaf.

Munsell Color Code: A system that describes colors based on threecharacteristics: hue, value (lightness), and chroma (color purity).

NDFD (Neutral Detergent Fiber Digestibility): Percentage of neutraldetergent fiber that is digestible; determined in vitro by incubating aground feed sample in live rumen fluid and measuring its disappearanceto simulate the amount and rate of digestion that would occur in therumen.

Number of Kernel Rows: Total number of kernel rows on the ear. If therows are indistinct, then this value is an average number of kernelslocated around the circumference of the ear at the mid-point of itslength.

Phenotype: Physical or external appearance of a plant or plant part.

Plant Height: Plant height in centimeters from the ground to the tip ofthe tassel.

Plots: Number of replicated plantings of a single hybrid that areharvested as data points for analyses in the same experiment.

Population: Number of seeds planted on a per acre basis.

Root Lodging: Percentage of corn plants that lean from the vertical axisat an approximate 30° angle or greater just before anthesis.

Silage moisture: Percentage moisture content of biomass measured atharvest.

Silk Color: Color of the silk three days after its emergence; recordedas standard color name [Light Green (1), Green-Yellow (5), Pale Yellow(6), Yellow (7), Salmon (9), Pink-Orange (10), Pink (11), Cherry Red(13), Purple (17), Tan (22)] and Munsell color code.

Stalk Lodging: Percentage of plants that have broken over at or belowthe top ear node at harvest.

Standard Color Names: Color names include Light Green (1), Medium Green(2), Dark Green (3), Very Dark Green (4), Green-Yellow (5), Pale Yellow(6), Yellow (7), Yellow-Orange (8), Salmon (9), Pink-Orange (10), Pink(11), Light Red (12), Cherry Red (13), Red (14), Red and White (15),Pale Purple (16), Purple (17), Colorless (18), White (19), White Capped(20), Buff (21), Tan (22), Brown (23), Bronze (24), Variegated (25), andOther (26).

Starch: Amount of starch carbohydrates; measured by near-infrared (NIR)spectroscopy as a percentage of dry silage matter.

Tassel Type: The tassel branch shape recorded as erect or spreading. Theangle of the base of each tassel branch is used to indicate thedirection of the branches. Erect longer or lighter tassels that droopover on the tip are classified as erect.

Tillers: Branches that develop from axillary buds at the base of a cornplant; they are morphologically identical to the main stalk and capableof forming their own root system, nodes, internodes, leaves, ears, andtassels. Recorded as present or absent.

Total tract dry matter degradability (TDMD): A compilation of all fiberfractions along the whole tract of the dairy animal; measured as sum ofruminal in situ and post-ruminal in vitro degradabilities as apercentage of dry matter.

Years: The number of calendar years of data included in a comparison.

II. Hybrid Corn Variety X21325

A. Hybrid Corn Variety X21325

In accordance with one aspect of the present invention, provided is anew yellow dent hybrid corn variety and plants thereof designatedX21325. Hybrid variety X21325 was produced from a cross of the inbredvarieties designated BE4207 and OOA20LY. The inbred parents have beenself-pollinated and ear-rowed a sufficient number of generations withcareful attention paid to uniformity of plant type to show uniformityand stability within the limits of environmental influence. Adescription of physiological and morphological characteristics of hybridcorn variety X21325 is presented in Table 1. It should be noted thatthese characteristics may have been measured on a trait bearing versionof hybrid corn variety X21325. However, one of ordinary skill in cornbreeding art would recognize that the measured characteristics would berepresentative of either the non-trait bearing version or atrait-bearing versions.

TABLE 1 Physiological and Morphological Characteristics of Hybrid 21325Characteristic Value STALK Plant Height (cm) 262 Ear Height (cm) 98Anthocyanin in Brace Roots (None, None Light, Moderate, Dark) Tillers(present/absent) Present LEAF Leaf Angle (Horizontal, Erect Semi-Erect,Erect) Leaf Color (standard) Dark Green (3) Leaves Above Ear (count) 9Ear-Node Leaf Width (cm) 10 Ear-Node Leaf Length (cm) 100 TASSEL TasselType (Spreading, Erect) Spreading Lateral Tassel Branches (count) 9Anther Color (standard) Pale Yellow (6) Glume Color (standard) LightGreen (1) EAR Silk Color (standard) Green-Yellow (5) Ear Leaves (Presnt,Absent) Present Ears Per Stalk (avg 5 plants) 1 Ear Length (cm) 19Number of Kernel Rows (count) 14 Kernel Row Type (Distinct, Indistinct)Distinct Kernel-Row Alignment (Straight, Straight Slightly Curved,Spiral) Ear Taper (Slight, Average, Extreme) Slight Cob Color (standard)Cherry Red (13) KERNEL Type (Flint or Dent) Dent Endosperm Type(description) Normal Starch Hard Endosperm Color (standard) Salmon (9)Kernel Crown Color (standard) Yellow (7)

It should be appreciated by one having ordinary skill in the art that,for the quantitative characteristics identified in Table 1, the valuespresented are typical values. These values may vary due to theenvironment and accordingly, other values that are substantiallyequivalent are also within the scope of the invention.

The present invention also relates to one or more corn plant parts ofhybrid corn variety X21325. Corn plant parts include plant cells, plantprotoplasts, plant cell tissue cultures from which corn plants can beregenerated, plant DNA, plant calli, plant clumps, and plant cells thatare intact in plants or parts of plants, such as embryos, pollen,ovules, flowers, seeds, kernels, ears, cobs, leaves, husks, stalks,roots, root tips, brace roots, lateral tassel branches, anthers,tassels, glumes, silks, tillers, and the like.

B. Hybrid Corn Variety Seed Designated X21325

A corn kernel is composed of four structural parts: (1) the pericarp,which is a protective outer covering (also known as bran or hull); (2)the germ (also known as an embryo); (3) the endosperm; and, (4) the tipcap, which is the point of attachment to the cob. Another aspect of thepresent invention is one or more parts of hybrid corn variety seedX21325, such as the pericarp or the germ and/or the endosperm whichremain upon removal of the pericarp and adhering remnants of the seedcoat.

Corn yield is affected by the conditions to which seeds and seedlings(young plants grown from seeds) are exposed. Seeds and seedlings may beexposed to one of, or a combination of, for example, cold, drought,salt, heat, pollutants, and disease, all of which are conditions thatpotentially retard or prevent the growth of crops therefrom. Forexample, temperature extremes are typical in the upper Midwest region ofthe United States. Furthermore, diseases evolved from pathogens anddeterioration caused by fungi are potentially harmful to seeds andseedlings. Thus, it is desirable to treat seeds as by coating orimpregnating the seeds with compositions that render the seeds andseedlings grown therefrom more hardy when exposed to such adverseconditions.

Accordingly, another aspect of the present invention relates to a coatedand/or impregnated seed of hybrid corn variety designated X21325 and tocoated and/or impregnated seed derived therefrom. Various agents havebeen used to treat seeds to increase resistance of the plants tostressed conditions, such as cold, drought, salt, and fungi. Such agentsinclude, for example, sodium methylphenyl-pentadienate, trichloroaceticacid, polyoxyalkylene-organo-siloxane block copolymer, 5-aminolevulinicacid, salicylic acid, thiamethoxam, potassium chloride, and polyvinylalcohol and are useful alone, or in combination in the presentinvention.

C. Deposit Information

A deposit of at least 2500 seeds of inbred parent plant varieties BE4207(U.S. Pat. No. 6,632,986, filed Jul. 18, 2001, the entire disclosure ofwhich is incorporated herein by reference) and OOA20LY (U.S. applicationSer. No. 15/171,340, filed Jun. 2, 2015, the entire disclosure of whichis incorporated herein by reference) has been made with the AmericanType Culture Collection (ATCC), 10801 University Boulevard, Manassas,Va. 20110-2209 USA, and assigned ATCC Accession Nos. PTA-5327, andPTA-122497, respectively. The seeds were deposited with the ATCC on Jul.18, 2001, and Sep. 4, 2015, respectively. Access to this deposit will beavailable during the pendency of the application to the Commissioner ofPatents and Trademarks and persons determined by the Commissioner to beentitled thereto upon request. The deposits will be maintained in theATCC Depository, which is a public depository, for a period of 30 years,or 5 years after the most recent request, or for the enforceable life ofthe patent, whichever is longer, and will be replaced if it becomesnonviable during that period. Applicant does not waive any infringementof their rights granted under this patent or under the Plant VarietyProtection Act (7 U.S.C. 2321 et seq.).

III. Processes of Preparing Novel Corn Plants

A. Novel Inbred Plants Obtained from Hybrid Corn Variety X21325

In accordance with processes of the present invention, hybrid cornvariety X21325 is crossed with itself or any different corn plant suchas an inbred corn plant or a hybrid corn plant to develop a novel inbredline. For example, hybrid corn variety X21325 may be inbred, i.e.,crossed to itself or sib-pollinated, and the resulting progeny eachselfed for about 5 to about 7 or more generations, thereby providing aset of distinct, pure-breeding inbred lines wherein each of the linesreceived all of its alleles from the hybrid corn plant. Double haploidmethods can also be used to obtain an inbred corn plant that ishomozygous at essentially every locus, wherein the inbred corn plantreceived all of its alleles from the hybrid corn plant. In otherembodiments, hybrid corn variety X21325 is crossed with a different cornplant that may include any inbred corn plant, another germplasm source,a haploid or mutation inducing stock, or a trait donor plant, therebyproviding a set of distinct, pure-breeding inbred lines. The resultinginbred lines could then be crossed with other inbred or non-inbred linesand the resulting inbred progeny analyzed for beneficialcharacteristics. In this way, novel inbred lines conferring desirablecharacteristics could be identified.

IV. Novel Hybrid Plants

A. Novel Hybrid Seeds and Plants

In yet another aspect of the invention, processes are provided forproducing hybrid corn variety X21325, which processes generally comprisecrossing a first parent corn plant BE4207 with a second parent cornplant OOA20LY. In these processes, crossing will result in theproduction of seed. The seed production occurs regardless whether theseed is collected.

Any time the inbred corn plant BE4207 is crossed with another, differentcorn inbred plant OOA20LY, a first generation (F₁) corn hybrid varietyX21325 plant is produced. Therefore, any F₁ hybrid corn plant or cornseed which is produced with these two parent corn lines is part of thepresent invention.

In embodiments of the present invention, the first step of “crossing”the first and the second parent corn plants comprises planting,preferably in pollinating proximity, seeds of a first inbred corn plantand a second, distinct inbred corn plant. As discussed herein, the seedsof the first inbred corn plant and/or the second inbred corn plant canbe treated with compositions that render the seeds and seedlings growntherefrom more hardy when exposed to adverse conditions.

A further step comprises cultivating or growing the seeds of the firstand second parent corn plants into plants that bear flowers. If theparental plants differ in timing of sexual maturity, techniques may beemployed to obtain an appropriate nick, i.e., to ensure the availabilityof pollen from the parent corn plant designated the male during the timeat which silks on the parent corn plant designated the female arereceptive to the pollen. Methods that may be employed to obtain thedesired nick include delaying the flowering of the faster maturingplant, such as, but not limited to delaying the planting of the fastermaturing seed, cutting or burning the top leaves of the faster maturingplant (without killing the plant) or speeding up the flowering of theslower maturing plant, such as by covering the slower maturing plantwith film designed to speed germination and growth or by cutting the tipof a young ear shoot to expose silk.

In a preferred embodiment, the corn plants are treated with one or moreagricultural chemicals as considered appropriate by the grower.

A subsequent step comprises preventing self-pollination orsib-pollination of the plants, i.e., preventing the silks of a plantfrom being fertilized by any plant of the same variety, including thesame plant. This is preferably done in large scale production bycontrolling the male fertility, e.g., treating the flowers so as toprevent pollen production or alternatively, using as the female parent amale sterile plant of the first or second parent corn plant (i.e.,treating or manipulating the flowers so as to prevent pollen production,to produce an emasculated parent corn plant or using as a female, acytoplasmic male sterile version of the corn plant). This control mayalso be accomplished in large scale production by physical removal ofthe tassel from the female plant, either by pulling the tassel by hand,cutting with a rotary cutter, or pulling with a mechanical tasselpulling machine. In small scale production, corn breeder's shoot bags,usually plastic or glassine, applied to cover the ear shoot prior to theextrusion of silks provide effective control of unwantedself-pollination or sib-pollination.

Yet another step comprises allowing cross-pollination to occur betweenthe first and second parent corn plants. When the plants are not inpollinating proximity, this is done by placing a bag, usually paper,over the tassels of the first plant and another shoot bag over the earshoot, prior to the extrusion of silk, of the incipient ear on thesecond plant. The bags are left in place usually overnight. Since pollenstops shedding each day and loses viability and new pollen is shed eachmorning, this assures that the silks are not pollinated from otherpollen sources, that any stray pollen on the tassels of the first plantis dead, and that the only pollen transferred comes from the firstplant. The pollen bag over the tassel of the first plant is then shakenvigorously to enhance release of pollen from the tassels and removedfrom the first plant. Finally, in one continuous motion, the shoot bagis removed from the silks of the incipient ear on the second plant, andthe pollen bag containing the captured pollen is placed over the silksof the incipient ear of the second plant, shaken again to disperse thecaptured pollen, and left in place covering the developing ear toprevent contamination from any unwanted fresh airborne pollen. In largescale production, crossing is accomplished by isolated open-pollinatedcrossing fields whereby corn plants of the parent designated as thefemale, which are controlled for male fertility, are allowed to bepollinated by other plants of a different corn type where such plantsare adjacent to the plants designated as the female parent.

A further step comprises harvesting the seeds, near or at maturity, fromthe ear of the plant that received the pollen. In a particularembodiment, seed is harvested from the female parent plant, and whendesired, the harvested seed can be grown to produce a first generation(F₁) hybrid corn plant.

Yet another step comprises drying and conditioning the seeds, includingthe treating, sizing (or grading) of seeds, and packaging for sale togrowers for the production of grain or forage. As with inbred seed, itmay be desirable to treat hybrid seeds with compositions that render theseeds and seedlings grown therefrom more hardy when exposed to adverseconditions. Mention should be made that resulting hybrid seed is sold togrowers for the production of grain and forage and not for breeding orseed production.

Still further, the present invention provides a hybrid corn plantproduced by growing the harvested seeds produced on the male-sterileplant as well as grain produced by the hybrid corn plant.

A single cross hybrid is produced when two different inbred parent cornplants are crossed to produce first generation F₁ hybrid progeny.Generally, each inbred parent corn plant has a genotype whichcomplements the genotype of the other inbred parent. Typically, the F₁progeny are more vigorous then the respective inbred parent corn plants.This hybrid vigor, or heterosis, is manifested in many polygenic traits,including markedly improved yields and improved stalks, roots,uniformity and insect and disease resistance. It is for this reason thatsingle cross F₁ hybrids are generally the most sought after hybrid. Athree-way, or modified single-cross hybrid is produced from three inbredlines (or synthetics) where two of the inbred lines are crossed (A×B)and then the resulting F₁ hybrid is crossed with the third inbred(A×B)×C, as where a modified female is used in the cross. A modifiedfemale provides an advantage of improved seed parent yield whereas amodified male improves pollen flow. A double cross hybrid is producedfrom four inbred lines crossed in pairs (A×B and C×D), thereby resultingin two F₁ hybrids that are crossed again. Double cross hybrids are morecommon in countries wherein less demand exists for higher yieldingsingle cross hybrids. Synthetic populations or crosses are developed bycrossing two or more inbred lines (or hybrids, or germplasm sources)together and then employing one of many possible techniques to randommate the progeny. Random mating the progeny is any process used by plantbreeders to make a series of crosses that will create a new germplasmpool from which new breeding lines can be derived. Much of the hybridvigor exhibited by F₁ hybrids is lost in the next generation (F₂).Consequently, seed from hybrids are not typically used for plantingstock.

B. Physical Description of F₁ Hybrids and F₁ Hybrid Comparison

During the development of a hybrid plant detailed evaluations of thephenotype are made including formal comparisons with other commerciallysuccessful hybrids. Because the corn is grown in close proximity,environmental factors that affect gene expression, such as moisture,temperature, sunlight, and pests, are minimized. For a decision to bemade to commercialize a hybrid, it is not necessary that the hybrid bebetter than all other hybrids. Rather, significant improvements must beshown in at least some traits that would create improvements in someniches. Examples of such comparative performance data for the hybridcorn plant X21325 are set forth herein below in Table 2.

TABLE 2 Hybrid 21325 Compared With Other Commercial Hybrids HybridMycogen Mycogen Characteristic 21325 TMF2L414 TMF2Q427 Biomass (tons/ac)26.4 23.8 24.1 Silage Moisture (%) 63.8 62.3 60.7 NDFD (%) 51.8 57.557.8 Starch (%) 27.1 30.2 32.4 TDMD (%) 76.1 80.4 80.8 Crude Protein (%)6.4 6.7 6.6 Plant Height (cm) 314.1 288.7 300.6 Ear Height (cm) 114.3103.2 130.7 Stalk Lodging (%) 0.1 0.5 0.2 Root Lodging (%) 2.5 0.1 −0.1Population (plants/ac) 33870 33050 33830 Environments (count) 27 26 25Years (count) 1 1 1 Plots/mean (count) 27 26 25

V. Novel X21325-Derived Plants

All plants produced using hybrid corn variety X21325 as a parent arewithin the scope of this invention. This includes plants essentiallyderived with the term “essentially derived variety” having the meaningascribed to such term in 7 U.S.C. § 2104(a)(3) of the Plant VarietyProtection Act, which definition is hereby incorporated by reference.This also includes progeny plant and parts thereof with at least oneancestor that is hybrid corn variety X21325 and more specifically wherethe pedigree of this progeny includes 1, 2, 3, 4, and/or 5 or crosspollinations to hybrid corn variety X21325, or a plant that has X21325as a progenitor. All breeders of ordinary skill in the art maintainpedigree records of their breeding programs. These pedigree recordscontain a detailed description of the breeding process, including alisting of all parental lines used in the breeding process andinformation on how such line was used. Thus, a breeder would know ifX21325 were used in the development of a progeny line, and would alsoknow how many breeding crosses to a line other than X21325 were made inthe development of any progeny line. A progeny line so developed maythen be used in crosses with other, different, corn inbreds to producefirst generation F₁ corn hybrid seeds and plants with superiorcharacteristics.

Accordingly, another aspect of the present invention is methods forproducing an inbred corn line X21325-derived corn plant. This method forproducing a X21325-derived corn plant, comprises: (a) crossing hybridcorn variety X21325 with a second corn plant to yield progeny corn seed;and, (b) growing the progeny corn seed, (under plant growth conditions),to yield the X21325-derived corn plant. Such methods may furthercomprise the steps of: (c) crossing the X21325-derived corn plant withitself or another corn plant to yield additional X21325-derived progenycorn seed; (b) growing the progeny corn seed of step (d) (under plantgrowing conditions), to yield additional X21325-derived corn plants; and(e) repeating the crossing and growing steps of (c) and (d) from 0 to 7times to generate further X21325-derived corn plants. Still further,this may comprise utilizing methods of haploid breeding and plant tissueculture methods to derive progeny of the X21325-derived corn plant.

VI. Corn Transformation

With the advent of molecular biological techniques that have allowed theisolation and characterization of genes that encode specific proteinproducts, scientists in the field of plant biology developed a stronginterest in engineering the genome of plants to contain and to expressforeign genes, or additional, or modified versions of native orendogenous genes (perhaps driven by different promoters) to alter thetraits of a plant in a specific manner. Such foreign, additional and/ormodified genes are referred to herein collectively as “transgenes.” Thepresent invention, in particular embodiments, also relates totransformed versions of the claimed hybrid corn variety X21325containing one or more transgenes.

Backcrossing methods can be used with the present invention to improveor introduce a trait in a hybrid via modification of its inbredparent(s). The term backcrossing as used herein refers to the repeatedcrossing of a hybrid progeny back to one of the parental corn plants forthat hybrid. The parental corn plant which contributes the locus or locifor the desired trait is termed the nonrecurrent or donor parent. Thisterminology refers to the fact that the nonrecurrent parent is used onetime in the backcross protocol and therefore does not recur. Theparental corn plant to which the locus or loci from the nonrecurrentparent are transferred is known as the recurrent parent as it is usedfor several rounds in the backcrossing protocol (Poehlman et al., 1995;Fehr, 1987; Sprague and Dudley, 1988).

In a typical backcross protocol, the original parent hybrid of interest(recurrent parent) is crossed to a second variety (nonrecurrent parent)that carries the genetic locus of interest to be transferred. Theresulting progeny from this cross are then crossed again to therecurrent parent and the process is repeated until a corn plant isobtained wherein essentially all of the desired morphological andphysiological characteristics of the recurrent parent are recovered inthe converted plant, in addition to the transferred locus from thenonrecurrent parent. The backcross process may be accelerated by the useof genetic markers, such as SSR, RFLP, SNP or AFLP markers to identifyplants with the greatest genetic complement from the recurrent parent.

The selection of a suitable recurrent parent is an important step for asuccessful backcrossing procedure. The goal of a backcross protocol isto add or substitute one or more new traits in the original inbred andhybrid progeny therefrom. To accomplish this, a genetic locus of therecurrent parent is modified or substituted with the desired locus fromthe nonrecurrent parent, while retaining essentially all of the rest ofthe desired genetic, and therefore the desired physiological andmorphological constitution of the original plant. The choice of theparticular nonrecurrent parent will depend on the purpose of thebackcross; one of the major purposes is to add some commerciallydesirable, agronomically important trait to the plant. The exactbackcrossing protocol will depend on the characteristic or trait beingaltered to determine an appropriate testing protocol. Althoughbackcrossing methods are simplified when the characteristic beingtransferred is a dominant allele, a recessive allele may also betransferred. In this instance it may be necessary to introduce a test ofthe progeny to determine if the desired characteristic has beensuccessfully transferred.

Many traits have been identified that are not regularly selected for inthe development of a new variety but that can be improved bybackcrossing techniques. A genetic locus conferring the traits may ormay not be transgenic. Examples of such traits known to those of skillin the art include, but are not limited to, male sterility, waxy starch,herbicide resistance, resistance for bacterial, fungal, or viraldisease, insect resistance, male fertility and enhanced nutritionalquality. These genes are generally inherited through the nucleus, butmay be inherited through the cytoplasm. Some known exceptions to thisare genes for male sterility, some of which are inheritedcytoplasmically, but still act as a single locus trait.

Direct selection may be applied where a genetic locus acts as a dominanttrait. An example of a dominant trait is the herbicide resistance trait.For this selection process, the progeny of the initial cross are sprayedwith the herbicide before the backcrossing. The spraying eliminates anyplants which do not have the desired herbicide resistancecharacteristic, and only those plants which have the herbicideresistance gene are used in the subsequent backcross. This process isthen repeated for all additional backcross generations.

It is understood to those of skill in the art that a transgene need notbe directly transformed into a plant, as techniques for the productionof stably transformed corn plants that pass single loci to progeny byMendelian inheritance is well known in the art. Such loci may thereforebe passed from parent plant to progeny plants by standard plant breedingtechniques that are well known in the art.

All publications, patents and patent applications mentioned in thespecification are indicative of the level of those skilled in the art towhich this invention pertains. All such publications, patents and patentapplications are incorporated by reference herein to the same extent asif each was specifically and individually indicated to be incorporatedby reference herein.

The foregoing invention has been described in some detail by way ofillustration and example for purposes of clarity and understanding.However, it should be appreciated by those having ordinary skill in theart that certain changes and modifications such as single genemodifications and mutations, somoclonal variants, variant individualsselected from large populations of the plants of the instant inbred andthe like may be practiced within the scope of the invention, as limitedonly by the scope of the appended claims, without departing from thetrue concept, spirit, and scope of the invention.

What is claimed is:
 1. A seed of the hybrid corn variety X21325,produced by crossing a first plant of variety BE4207 with a second plantof variety OOA20LY, wherein representative seed of said varieties BE4207and OOA20LY have been deposited under ATCC Accession numbers PTA-5327and PTA-122497, respectively.
 2. A plant of the hybrid corn varietyX21325 grown from the seed of claim
 1. 3. A plant part of the plant ofclaim 2, the plant part comprising at least one cell of hybrid cornvariety X21325.
 4. The seed of claim 1, wherein one or both of the firstand second plants further comprises a transgene.
 5. The seed of claim 4,wherein the transgene confers a trait selected from the group consistingof male sterility, herbicide tolerance, insect resistance, diseaseresistance, modified fatty acid metabolism, modified phytic acidmetabolism, modified carbohydrate metabolism and modified proteinmetabolism.
 6. The seed of claim 4, wherein the first and second plantseach comprise a different transgene.
 7. The seed of claim 4, wherein oneor both of the first and second plants comprises a single locusconversion.
 8. The seed of claim 7, wherein the single locus conversionconfers a trait selected from the group consisting of male sterility,herbicide tolerance, insect resistance, disease resistance, modifiedfatty acid metabolism, modified phytic acid metabolism, modifiedcarbohydrate metabolism and modified protein metabolism.
 9. The methodof producing hybrid corn seed comprising crossing a plant of varietyBE4207 with a plant of variety OOA20LY, wherein representative seed ofvariety BE4207 and variety OOA20LY have been deposited under ATCCAccession numbers PTA-5327 and PTA-122497, respectively.
 10. The methodof claim 9, defined as comprising pollinating a plant of inbred varietyBE4207 with pollen from a plant of variety OOA20LY.
 11. The method forproducing corn grain comprising growing the plant of claim 2 until grainis produced and collecting the grain.
 12. The method of introducing aheritable trait into hybrid corn variety X21325 comprising the steps of:(a) crossing a first plant of a first inbred corn variety selected fromthe group consisting of variety BE4207 and variety OOA20LY with anothercorn plant that heritably carries the trait to produce progeny plants,at least some of which heritably carry the trait, wherein representativesamples of seed of variety BE4207 and variety OOA20LY have beendeposited under ATCC Accession numbers PTA-5327 and PTA-122497,respectively; (b) selecting progeny plants that heritably carry thetrait; (c) crossing selected progeny plants with another plant of thefirst inbred corn variety to produce next-generation progeny plants atleast some of which heritably carry the trait; (d) selectingnext-generation progeny plants that heritably carry the trait andexhibit morphological and physiological characteristics of the firstinbred corn variety; (e) repeating steps (c) and (d) three or more timesto produce at least a first selected progeny plant that heritablycarries the trait and exhibits all of the morphological and physicalcharacteristics of the inbred corn variety; and (f) crossing a progenyplant of step (e) with a plant of the other inbred corn variety of thegroup consisting of BE4207 and OOA20LY to produce a plant comprising thetrait and all of the characteristics of hybrid corn variety X21325 whengrown under the same environmental conditions.
 13. The method of claim12, wherein the trait is selected from the group consisting of malesterility, herbicide tolerance, insect resistance, disease resistance,waxy starch, modified fatty acid metabolism, modified phytic acidmetabolism, modified carbohydrate metabolism and modified proteinmetabolism.
 14. The method of claim 13, further comprising repeatingsteps (a)-(f) at least once to introduce at least a second trait intohybrid corn variety X21325, wherein the second trait is selected fromthe group consisting of male sterility, herbicide tolerance, insectresistance, disease resistance, waxy starch, modified fatty acidmetabolism, modified phytic acid metabolism, modified carbohydratemetabolism and modified protein metabolism.
 15. A plant produced by themethod of claim
 12. 16. A method of introducing a desired trait intohybrid corn variety X21325 comprising the steps of: (a) introducing atransgene conferring the trait into a variety selected from the groupconsisting of BE4207 and OOA20LY to produce a transgenic plant heritablycarrying the trait; and (b) crossing the transgenic plant or an isogenicprogeny plant thereof with a plant of the other inbred corn variety toproduce seed of the hybrid corn variety X21325 that heritably carriesand expresses the transgene and otherwise has essentially all of themorphological and physiological characteristics of hybrid corn varietyX21325 when grown under the same environmental conditions.
 17. Themethod of claim 16, wherein the desired trait is selected from the groupconsisting of male sterility, herbicide tolerance, insect resistance,disease resistance, waxy starch, modified fatty acid metabolism,modified phytic acid metabolism, modified carbohydrate metabolism andmodified protein metabolism.
 18. The method of claim 16, furthercomprising repeating steps (a) and (b) at least once to introduce atleast a second trait into hybrid corn variety X21325, wherein the secondtrait is selected from the group consisting of male sterility, herbicidetolerance, insect resistance, disease resistance, waxy starch, modifiedfatty acid metabolism, modified phytic acid metabolism, modifiedcarbohydrate metabolism and modified protein metabolism.
 19. A plantproduced by the method of claim
 18. 20. A method of producing a cornplant derived from the hybrid corn variety X21325, comprising crossingthe plant of claim 2 with a second corn plant to produce a progeny cornplant derived from the hybrid corn variety X21325.